Correlation of molecular and conventional methods of diagnosis in tuberculous lymphadenitis
نویسندگان
چکیده
Methods Sixty lymph node aspirates from suspected cases of tuberculous lymphadenitis were examined for Mycobacterium tuberculosis by Ziehl neelsen (ZN) staining, culture on a Lowenstein Jensen medium, and cytological examination for findings suggestive of tubercular lymphadenitis. DNA from all the samples was amplified by conventional PCR targeting 123 bp and 240 bp fragments of IS6110 and MPT64 genes respectively and RealTime PCR targeting MPT64 gene only.
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